Reducing basal nitrogen rate to improve maize seedling growth, water and nitrogen use efficiencies under drought stress by optimizing root morphology and distribution

Abstract

Frequent spring droughts and nitrogen (N) fertilizer overuse limit rain-fed maize production in Northeast China. However, the interactions between water-stress and N rates on maize seedling growth and root development remain elusive. In this study, a pot experiment was conducted with maize grown under three N rates (N omission, low and high N rates, i.e., N0, LN and HN) and severe water-stress (SS), moderate water-stress (MS) and well-watered (WW) conditions for 2-week from 4-leaf stage. Dry biomass (DM), leaf rolling performance, water use efficiency (WUE), N fertilizer use efficiency (NUE), root morphology and distribution were evaluated. It was showed that soil water levels and N rates had significant individual and interactive effects on most measured parameters. The dual stress of drought and N deficiency severely limited maize growth and N uptake. Nitrogen fertilization improved maize growth, N uptake and WUE under water-stress. However, compared with HN treatment, LN treatment had less water consumption, higher leaf relative water content and less leaf rolling symptom both under the MS and SS conditions. The reason is that LN enhanced root growth and elongation with more fine roots, especially in deep soil. Notably, maize plants in MS-LN treatment had an optimal root distribution characterized by higher root length density (0.30 cm cm−3), larger and deeper penetration scale throughout the soil layers, and thus showed fewer drought responses and obtained the highest WUE (4.1 g L−1) and NUE (17.2%) among all the water-stress treatments. In contrast, HN limited root growth and extension in deep soil and in turn increased water and N depletion, resulting in more serve leaf rolling and lower WUE and NUE. Therefore, reducing basal N rate is recommended to optimize root growth, morphology and distribution at the seedling stage in rain-fed maize production, to enhance drought resistance and improve WUE and NUE.