Abstract
The G-quadruplex–hemin DNAzyme has shown potential as a new catalytic label for the development of various biosensors. But the typical problem of high background induced by excess hemin itself limits the application of the method. In this work, we found that single-walled carbon nanotubes (SWNTs) can effectively decrease the background of G-quadruplex–hemin DNAzyme sensing platform. The method relies upon the strong affinity of SWNTs with hemin. In the presence of SWNTs, superfluous hemin is arrested the surface of SWNTs, and then is removed though centrifugation It was proved that SWNTs reduce the background of G-quadruplex–hemin DNAzyme sensing platform by ABTS–H 2O 2 colorimetric. Moreover, we detected K + with the AGRO100 as the sensing element. The present limit of detection for K + is 2 nM, and the sensitivity for analyzing K + is improved by about 10-fold as compared with the reported colorimetric counterpart in the absence of SWNTs.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.