Abstract
COVID-19 has been widely spreading. We aimed to examine adaptive immune cells in non-severe patients with persistent SARS-CoV-2 shedding. 37 non-severe patients with persistent SARS-CoV-2 presence that were transferred to Zhongnan hospital of Wuhan University were retrospectively recruited to the PP (persistently positive) group, which was further allocated to PPP group (n = 19) and PPN group (n = 18), according to their testing results after 7 days (N = negative). Epidemiological, demographic, clinical and laboratory data were collected and analyzed. Data from age- and sex-matched non-severe patients at disease onset (PA [positive on admission] patients, n = 37), and lymphocyte subpopulation measurements from matched 54 healthy subjects were extracted for comparison (HC). Compared with PA patients, PP patients had much improved laboratory findings. The absolute numbers of CD3+ T cells, CD4+ T cells, and NK cells were significantly higher in PP group than that in PA group, and were comparable to that in healthy controls. PPP subgroup had markedly reduced B cells and T cells compared to PPN group and healthy subjects. Finally, paired results of these lymphocyte subpopulations from 10 PPN patients demonstrated that the number of T cells and B cells significantly increased when the SARS-CoV-2 tests turned negative. Persistent SARS-CoV-2 presence in non-severe COVID-19 patients is associated with reduced numbers of adaptive immune cells. Monitoring lymphocyte subpopulations could be clinically meaningful in identifying fully recovered COVID-19 patients.
Highlights
Emerged in Wuhan, China in December 2019, COVID-19 has quickly spread to other provinces of China, and to 170 other countries and areas across six continents
37 non-severe COVID-19 patients that were tested positive for SARS-CoV-2 more than 20 days were recruited to the PP group
We were able to extract paired results of lymphocyte subpopulations for 10 patients on admission, and on the first day they tested negative for viral RNA. These patients demonstrated markedly increased C D3+ T cells (p = 0.001), C D4+ T cells (p = 0.002), C D8+ T cells (p = 0.009), and B cells (p = 0.008) after turned negative for SARS-CoV-2 (Fig. 2). These results indicated that the abnormalities in adaptive immune cells, but not symptoms and laboratory indicators, were associated with SARS-CoV-2 viral RNA detection in non-severe COVID-19 patients
Summary
Emerged in Wuhan, China in December 2019, COVID-19 has quickly spread to other provinces of China, and to 170 other countries and areas across six continents. Despite the high mortality rate of 4.2% worldwide, around 85% of patients infected with SAR-CoV-2 developed non-severe disease, as recently reported by two large epidemiological s tudies[2,3]. These patients appeared to have a better clinical outcome compared to those with severe d isease[2]. This study was designed to examine the correlation of changes in adaptive immune cells with persistent viral RNA presence in non-severe COVID-19 patients, and to evaluate its potential application in discharge management for these patients
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