Reduced gravitropic sensitivity in roots of a starch-deficient mutant ofNicotiana sylvestris

Abstract

Gravitropism was studied in seedlings ofNicotiana sylvestris Speg. et Comes wild-type (WT) and mutant NS 458 which has a defective plastid phosphoglucomutase (EC 2.7.5.1). Starch was greatly reduced in NS 458 compared to the WT, but small amounts of starch were detected in root-cap columella cells in NS 458 by light and electron microscopy. The roots of WT are more sensitive to gravity than mutant NS 458 roots since: (1) in mutant roots, curvature was reduced and delayed in the time course of curvature; (2) curvature of mutant roots was 24-56% that of WT roots over the range of induction periods tested; (3) in intermittent-stimulation experiments, curvature of mutant roots was 37% or less than that of WT roots in all treatments tested. The perception time, roots in all treatments tested. The perception time, determined by intermittent-stimulation experiments, was ≤5 s for WT roots and 30-60 s for mutant roots. The growth rates for WT and NS 458 roots were essentially equal. These results and our previous results with WT and starchless mutantArabidopsis roots (Kiss et al. 1989, Planta177, 198-206) support the conclusions that a full complement of starch is necessary for full gravitropic sensitivity and that amyloplasts function in gravity perception. Since a presumed relatively small increase in plastid buoyant mass (N. sylvestris mutant versusArabidopsis mutant) significantly improves the orientation of theN. sylvestris mutant roots, we suggest that plastids are the likeliest candidates to be triggering gravity perception in roots of both mutants.