Abstract
Reduced graphene oxide (rGO) is synthesized using strong oxidizing agents with the help of chemical reduction method. Structural and physiochemical properties of the prepared sample are studied by ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). UV-Vis spectra of GO exhibit maximum absorption peak attributable to restoration of sp 2carbon. Electrochemical study performed for bare and rGO modified glassy carbon electrode. Electrochemical sensing of Dopamine (DA) and Uric Acid (UA) was examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were conducted in phosphate buffer solution (PBS) at pH = 7.0 done for both simultaneous and individual determination of DA and UA in the range of 500nM – 1mM exhibit a wide linear range of detection and having low limit of detection (LOD) of 0.67µM for DA, and 0.69µM for UA estimated for simultaneous determination and in individual determination it was found to be 0.51µM and 0.42µM for DA and UA respectively. Additionally, the modified electrode exhibits good reproducibility, selectivity, sensitivity and stability. The proposed sensor could be applied for the determination of DA and UA in real samples.
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