Reduced graphene oxide as a resonance light-scattering probe for thrombin detection using dual-aptamer-based dsDNA

Abstract

This paper describes a reduced graphene oxide (RGO)-based resonance light-scattering (RLS) method for thrombin detection by using double strand DNA (dsDNA) as a binding element. dsDNA is obtained by hybridizing DNA1 and DNA2, which respectively consist of one aptamer of thrombin and the complementary strand of the other aptamer of thrombin. When thrombin is added, the specific binding of two aptamers to thrombin results in a complex (DNA1–thrombin–DNA2) and triggers the release of the complementary strand of two aptamers from dsDNA. The released ssDNA can be self-assembled on the surface of RGO to form a stable DNA1–thrombin–DNA2–RGO complex, which increases RLS signals. This simple and rapid method has enabled the detection of thrombin in the picomolar level in buffer and human serum samples. This study is the first to use RGO as a platform in RLS sensor, which can extend the application of RGO.