Year-end Sale % OFF 
Abstract
AbstractOver the last few years, good quality freezable semen was not utilized effectively due to less knowledge about the freezability of semen. One reason is the lack of sufficient antioxidants in the seminal plasma and semen extender. The antioxidant content is reduced during the cryopreservation process and causes premature cryo-capacitation and modification of sperm membrane structure upon thawing. So the study of pre-freezing and post thaw seminal parameters like viability, motility, acrosomal integrity, lipid peroxide assay, vanguard distance traveled by sperm, mitochondrial membrane potential, velocity and motility parameters (CASA) and field fertility trial helped to assess the effectiveness of the additives. The conception rate was better in reduced glutathione than the cysteine hydrochloride treated group. The glutathione has improved the poor freezing and maintained the good freezable semen. Thus the field fertility rate was enhanced and in turn helped to prevent waste of good quality germplasm and repeat breeder syndrome in cows.
Highlights
Artificial insemination is the first generation biotechnological advancement that has made a profound contribution to the genetic improvement, in dairy bulls through which a single ejaculate from male is used to impregnate many females
In the present investigation three Good and three Poor freezable crossbred Jersey bulls belonging to Frozen Semen Bank, Cuttack, Government of Orissa were selected and six ejaculates from each bull diluted with Tris extender with out additives was considered as Control (Group I) incorporation of additives either reduced Glutathione (Group III) or Cysteine hydrochloride (Group II) as semen additive before cryo preservation at -1960C in liquid nitrogen was taken up
The efficacy of semen additives like Cysteine hydrochloride and Glutathione were assessed on the basis of seminal traits and the resultant conception rate following artificial insemination
Summary
Artificial insemination is the first generation biotechnological advancement that has made a profound contribution to the genetic improvement, in dairy bulls through which a single ejaculate from male is used to impregnate many females This impact could not have been possible without long standing freezing of bull semen. Long term storage facilitates semen transport over distances and enables extended use of superior germplasm, even after the sire’s death This has attained a status of industry especially for the dairy animals due to wide use of frozen semen, but as yet semen cryopreservation could not attain status of reality because of the fact that following freezing and thawing a large number of sperms are rendered incapable to fertilize the ovum. A variety of additives like anti-oxidants, membrane stabilizers, motility enhancers and chelating agents have been used to protect spermatozoa from deleterious effects of cryopreservation and for improving freezability and fertility of bull semen
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
