Abstract

GmRAV-transgenic tobacco plants exhibited decreased gibberellins (GAs) biosynthesis related phenotypes, including dwarfism and repressed flowering time. The dwarf phenotype of GmRAV-overexpressing (ox) tobaccos was fully restored in stem and hypocotyl length of transgenic plants by the application of exogenous GA3. The epidermal cells in the stem internodes of the GmRAV-ox tobacco plants treated with GA3 were obviously the same as those in wild-type plants in longitudinal direction. Therefore, the rescued plant height of GmRAV-ox tobaccos by GA3 might be largely caused by increased cell size. GmRAV overexpression inhibited GA biosynthesis due to a transcriptional repression of GA20ox gene. The dwarfed phenotype of GmRAV-ox tobaccos with reduced stem elongation could be caused by a reduction in GA3 content.

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