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Abstract
A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10–750 μM with a limit of detection (LOD) of about 1 μM. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.
Highlights
The determination of reactive oxygen and nitrogen species (RONS) is the object of study of numerous physiological and pathological processes [1,2]
The objective of this paper is to describe a simple and inexpensive method for nitric oxide (NO) detection and quantification based on the fluorescence development resulting from the reaction of NO with non-fluorescent reduced fluoresceinamine
Preparation of the reduced fluoresceinamine aqueous solution is straightforward since it is the direct product of the reaction between fluoresceinamine and solid zinc in acid medium, and the reduction can be visually monitored because the unreduced chemical form is bright yellow and the reduced form has no color
Summary
The determination of reactive oxygen and nitrogen species (RONS) is the object of study of numerous physiological and pathological processes [1,2]. The production of RONS, which includes nitric oxide (NO), is mediated by multiple oxidative stress mechanisms responsible for changes in the Sensors 2010, 10 cells and tissues [3,4,5,6]. Beside this role in these pathological processes, RONS compounds are involved in numerous physiological process of regulation in the organism [7,8]. NO is well-known as a vasodilator and as an inhibitor of platelet activation and aggregation [9]. The development of advanced analytical tools with the ability to detect
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