Reduced cytokine production by glycogen‐elicited peritoneal cells from diabetic rats.

Abstract

Studies with diabetic rats and mice showed important alterations in neutrophil function such as decreased cell migration, phagocytosis capacity and release of TNF‐α, IL‐1β and PGE2 upon exposure to LPS. Elicited peritoneal cells (mainly neutrophils) obtained from STZ‐induced diabetic male Wistar rats (65mg/Kg b.w, 2 and 6 weeks) were investigated. Diabetic (D) and control (C) rats received an intraperitoneal injection of glycogen solution (1%). Four hours afterwards, the peritoneal lavage was performed and the following parameters were: total cell counts in peritoneal lavage and the measurement of the cytokines (IL‐1β, CINC‐2α/β, TNF‐α and IL‐10) was performed in samples of the peritoneal cavity fluid by ELISA. A similar number of cells migrated to the peritoneal cavity in all groups. The diabetic state caused a significant decrease of IL‐1β (45%), TNF‐α (47%), IL‐10 (50%) and CINC‐2α/β (55%) levels in the intraperitoneal cavity fluid. However, the amount of cytokine produced by elicited peritoneal cells from insulin treated diabetic rats was the same observed in control rats. Therefore, the impairment in the production/liberation of cytokines by elicited peritoneal cells might contribute to an impaired inflammatory response and high susceptibilityto infections observed in diabetic state.Research supported by: FAPESP and CNPq