REDOX Responsive Fluorescence Active Glycopolymer Based Nanogel: A Potential Material for Targeted Anticancer Drug Delivery.

Abstract

A well-defined glycopolymer based fluorescence active nanogel has been prepared via the combination of reversible addition-fragmentation chain transfer (RAFT) polymerization and Diels-Alder (DA) "click" chemistry. To prepare the nanogel, initially, a functional AB block copolymer (BCP) poly(pentafluorophenyl acrylate)-b-poly(furfuryl methacrylate) (PPFPA-b-PFMA), having activated pentafluorophenyl ester group, was synthesized via RAFT polymerization. The activated pentafluorophenyl functionality was replaced by the amine functionality of glucosamine to introduce the amphiphilic BCP poly[2-(acrylamido) glucopyranose]-b-poly(furfuryl methacrylate) (PAG-b-PFMA). Furthermore, the terminal acid (-COOH) functionality of the RAFT agent was modified by gelatin QDs (GQDs) to generate fluorescence active glycopolymer. An anticancer drug, Doxorubicin, was loaded in the micelle via the successive addition of the drug molecule and cross-linking using dithio-bismaleimidoethane (DTME), a REDOX responsive cross-linker. The anticancer activity of the drug loaded nanogel was observed over MBA-MD-231, human breast cancer cell line, and monitored via fluorescence spectroscopy and flow cytometric analyses (FACS). The cytotoxicity of the prepared glycopolymer based nanogel over the MBA-MD-231 cell line was assessed via MTT assay test, and it was observed that the synthesized nanogel was noncytotoxic in nature.