Redox-regulation and anti-inflammatory system activation by quercetin-3-O-β-D-glucopyranoside-rich fraction from Spondias mombin leaves: biochemical, reproductive and histological study in rat model of dichlorvos toxicity

Abstract

Abstract Objective This study was designed to investigate the redox-regulation capacity of Spondias mombin leaf fraction rich in Quercetin-3-O-β-D-glucopyranoside (Q3G-RF) in rat model of dichlorvos (DDVP) toxicity. Method Male Wistar rats randomly allotted to six groups with 12 rats each were administered appropriate regimens orally – sunflower oil, Q3G-RF (100 mg/kg), dichlorvos (8.8 mg/kg), Q3G-RF (100 mg/kg) + dichlorvos (8.8 mg/kg), Q3G-RF (50 mg/kg) + dichlorvos (8.8 mg/kg), and Q3G-RF (100 mg/kg) + clomid (0.35 mg/kg) for groups 1, 2, 3, 4, 5, and 6 respectively. Key findings DDVP reduced the SOD, CAT, GST, GPx, GR, QR, T-SH, GSH, TAC; ALP, ACP, glucose, sialic acid, 3- and 17-β-HSD, TNF-α, IL-6, IL-10, IL-1β, NO, MPO, caspase 3; sperm functions; testosterone, FSH, LH, estradiol, testosterone/estradiol ratio; organ-body weight, body weight, without altering semen volume and semen pH but increased the morphological abnormalities number of sperm cells in head, neck, and tail; level of lipid peroxidation, cholesterol and H2O2 relative to control animals. Co-administration with Q3G-RF or clomid revoked dichlorvos effect relative to the control. Conclusion Q3G-RF reversed the induced oxidative stress and inflammation as well as suppressed sperm function and reproductive parameters caused by dichlorvos, suggesting the exploration as a therapeutic agent in managing male fertility disorders.