Redox proteomic study of Bacillus cereus thiol proteome during fermentative anaerobic growth

Fella Hamitouche,Catherine Duport,Philippe Schmitt,Jean Armengaud,Jean-Charles Gaillard,Luc Dedieu

doi:10.1186/s12864-021-07962-y

Abstract

BackgroundBacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown under fermentative anoxic conditions. We used a quantitative thiol trapping method combined with proteomics profiling.ResultsIn total, we identified 153 reactive Cys residues in 117 proteins participating in various cellular processes and metabolic pathways, including translation, carbohydrate metabolism, and stress response. Of these reactive Cys, 72 were detected as reduced Cys. The B. cereus Cys proteome evolved during growth both in terms of the number of reduced Cys and the Cys-containing proteins identified, reflecting its growth-phase-dependence. Interestingly, the reduced status of the B. cereus thiol proteome increased during growth, concomitantly to the decrease of extracellular oxidoreduction potential.ConclusionsTaken together, our data show that the B. cereus Cys proteome during unstressed fermentative anaerobic growth is a dynamic entity and provide an important foundation for future redox proteomic studies in B. cereus and other organisms.

Highlights

Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions
Our results show that the B. cereus thiol proteome is growth-phase-dependent and contains higher number of reduced Cys in the later stages of growth
All proteins were digested with trypsin, and the resulting peptides were submitted to extensive LC-MS/ MS analysis

Summary

Introduction

Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. As well as being a facultative anaerobe, B. cereus can adapt to a wide range of environmental conditions allowing it to multiply in a number of food products and in the human intestine [2, 3]. In these environments, B. cereus adapts its metabolism to variations in temperature [4], pH, oxygen level, and oxidoreduction potential (ORP). NAD+/ NADH is central to catabolism and energy supply, whereas the NADP+/ NADPH couple plays an important role in biosynthesis and detoxification of cells [7].

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