Redox Behavior of Anticancer Chalcone on a Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

Afzal Shah,Syed S Shah,Muhammad F Nazar,Asad M Khan,Farzana L Ansari,Rumana Qureshi

doi:10.3390/ijms9081424

Afzal Shah, Syed S Shah + Show 4 more

Open Access

https://doi.org/10.3390/ijms9081424

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Abstract

The interaction of anticancer chalcone [AMC, 1-(4′-aminophenyl)-3-(4-N,N-dimethylphenyl)-2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC –DNA intercalation. A binding constant (K) with a value of 6.15 × 105 M−1 was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (Db and Du), heterogeneous electron transfer rate constant (ko) and electron affinity (A) were also calculated from electrochemical data.

Highlights

IntroductionCancer is the secondmost leading cause of death after heart attack
Due to ineffective drugs, cancer is the secondmost leading cause of death after heart attack.the researchers have accelerated their efforts for the generation of new anticancer drugs with high therapeutic index
Its CV, UV-Vis and vicometric results confirmed an intercalative mode of binding with high affinity for DNA as is clear from its binding constant of 2.86 × 105 M-1

Summary

Introduction

Cancer is the secondmost leading cause of death after heart attack. The researchers have accelerated their efforts for the generation of new anticancer drugs with high therapeutic index. In this connection, a clinically effective antitumor derivative of chalcone. Several research teams have already reported the interaction of compounds and DNA using conventional three electrode cells which consisted of GCE, SCE and Pt wire. These works provide much valuable information for further investigation and the design of new anticancer drugs [13,14].

Redox studies of AMC-DNA interaction

Spectroscopic studies

Viscosity measurements

Reagents and chemicals

Apparatus and procedures

Conclusions