Rediscovery of Gastrostyla setifera (Engelmann, 1862) Kent, 1882: morphology and divisional morphogenesis (Ciliophora, Hypotrichida)

Abstract

The morphology and morphogenesis of the poorly known hypotrichous ciliate Gastrostyla setifera, isolated from a freshwater pond near Harbin, China, are described from both living and protargol silver-impregnated specimens. The Harbin population of this species is characterized thus: medium-sized freshwater Gastrostyla, 150-205 × 70-95 μm in vivo, ellipsoid in shape but somewhat narrowed posteriorly. Contractile vacuole located in left half of mid-body region. On average 48-56 (mean 52) adoral membranelles; 10 frontal and 9-11 ventral cirri; 23-26 left and 30-34 right marginal cirri; usually with four complete dorsal kineties and two dorsomarginal kineties; three caudal cirri present at posterior margin; invariably two macronuclear nodules and two micronuclei. Its morphogenesis reveals the following characteristics: (1) adoral zone of membranelles (AZM) in the opisthe arises from the new oral primordium while the proter inherits the entire parental structure; (2) 10 frontal, 9-11 ventral and five transverse cirri are derived from five frontoventral transverse cirral anlagen together with the undulating membrane anlage; (3) left and right marginal cirri are derived from new anlagen which originate within the old marginal rows; (4) the generation of the dorsal kineties is of the 'two-group-mode'; three left primary dorsal anlagen and two dorsomarginal anlagen appear as two groups in both dividers from which all dorsal kineties are derived (the fourth kinety is formed due to the fragmentation of the third anlage); (5) one caudal cirrus is formed at the ends of each of the first, second and fourth dorsal anlagen.