Abstract
AbstractThis study investigates the hypothesis that the ethyl acetate extract of rice fermented with red yeast (Monascuspilosus) in medium containing garlic (RYRGE) inhibits the differentiation of 3T3‐L1 preadipocytes by down regulating cellular induction of PPARγ, and C/EBPα. Mouse 3T3‐L1 preadipocytes were treated with 0.0, 0.125, and 0.25 mg/mL of RYRGE, and Oil Red O staining, triglyceride accumulation and glyceradehyde‐3‐phosphate dehydrogenase (GPDH) activity were measured as markers of adipocyte differentiation. The protein and mRNA expressions of PPARγ and C/EBPα were measured using Western Blot and RT‐PCR, respectively. The mRNA expressions of leptin and aP2 genes were measured using RT‐PCR. RYRGE significantly inhibited lipid accumulation and GPDH activity in dose‐dependent manner (p<0.05). The mRNA and protein expressions of PPARγ and C/EBPα were dose‐dependently inhibited by RYRGE. Similarly, RYRGE decreased the gene expression of adipocyte fatty acid binding protein (aP2) and leptin in a dose‐dependent manner (p<0.05), which generally acts as an adipogenic marker proteins and C/EBPα and PPARγ target genes. These results indicate that RYRGE effectively attenuated adipogenesis by down‐regulating the expression of adipocyte transcription factors and related adipogenic gene expressions.
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