Red propolis and L-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells.

Camila De Carvalho Juanes,Vanessa Nogueira Lages Braga,Francisco Stefânio Barreto,Francisco Vagnaldo Fechine,Conceição Aparecida Dornelas,Gisele Rocha Aguiar,Kleison Douglas Gomes Pimentel,Susana Moreira De Souza

doi:10.31744/einstein_journal/2019ao4576

Camila De Carvalho Juanes, Vanessa Nogueira Lages Braga + Show 6 more

Open Access

https://doi.org/10.31744/einstein_journal/2019ao4576

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Abstract

ABSTRACTObjective:To evaluate the effect of red propolis and L-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells.Methods:The study consisted of two experiments with four groups each (total: 57 hamsters). In the experiment 1, the animals were inoculated with Walker tumor cells, followed by administration of test substances (red propolis 200mg/5mL/kg or L-lysine 150mg/kg) or control substances (gum arabic 5mL/kg or water 5mL/kg) for 10 days. The animals in the experiment 2 received red propolis, L-lysine, gum arabic or water at the same doses, for 33 days prior to inoculation of Walker tumor cells, followed by 10 days of treatment with the same substances. Based on single-plane images, angiogenesis was quantified (mean vascular area), in percentage, and tumor area (mm2) and perimeter (mm).Results:In the experiment 1, compared to animals receiving water, the mean vascular area expressed in percentage was significantly smaller in animal treated with propolis (p<0.05) and L-lysine (p<0.001).Conclusion:Both red propolis and L-lysine inhibited tumor angiogenesis in the new hamster cheek pouch model when administered after tumor inoculation.

Highlights

Angiogenesis is the formation of new blood vessels from existing ones.[1]. In 1970, Folkman opened up new perspectives for cancer therapy suggesting that tumor growth was related to and dependent on neovascularization
The ensuing discovery of the first endogenous angiogenesis inhibitors confirmed his hypothesis, and gave rise to a frantic search for new models to study angiogenesis and antiangiogenic compounds among the molecules known to be present in biodiversity products
Walker tumor cells have been used in several tumor implant models.[8]. The cheek pouch of hamster (Mesocricetus auratus)(9,10) is a tissue suitable for a new model, since the membrane enables visualizing vessels

Summary

Introduction

Angiogenesis (or neovascularization) is the formation of new blood vessels from existing ones.[1]. Walker tumor cells have been used in several tumor implant models.[8] The cheek pouch of hamster (Mesocricetus auratus)(9,10) is a tissue suitable for a new model, since the membrane enables visualizing vessels. The hamster cheek pouch implant model described in the literature inoculated tumor fragments, not cells.[9] In our experimental model, a standardized number of tumor cells were inoculated to achieve greater consistency of tumor growth between individuals, yielding more reliable results. Complement, lymphocyte and macrophage activation has been observed in many studies, suggesting they are part of the mechanism by which propolis induces apoptosis in tumor cells.[11,12,13] In this way, we included an experiment with 33 days of treatment with propolis prior to inoculation (experiment 2)

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Results

Conclusion