Abstract
The effect of nutrient substances upon the production of red pigments by interaction of Penicillium verruculosum L 9 with Trichoderma viride L 6 were investigated and the following results were obtained.A medium containing 1.0% of glucose, 1.0% of Difco-peptone and adjusted pH to 6.0 was found to be the best as a non-synthetic medium. Among a number of carbonic materials tested (hexoses, pentoses, disaccharides, polyalcohols, organic acids, and alcohols) only mannose and malic acid gave positive results comparable to that observed with glucose. No pigment formation took place when inorganic nitrogenous materials such as NH4OH, NH4Cl, (NH4)2SO4, NH4NO3 and (NH4)2CO3 were added as a sole nitrogen source to the semi-synthetic medium. The red pigment formation comparable to that obtained with the glucose-peptone medium could be induced when 1.0% of either casamino acid, urea, L-arginine HCl or DL-proline was added as a nitrogen source to a medium consisting of 4% glucose, 0.1% MgSO4.7H2O, 0.1% MnSO4.4H2O, 0.5% KH2PO4 and 0.01% ZnSO4. The concentration of L-arginine HCl and of DL-proline in theab ove-mentioned medium, which caused maximum formation of the red pigment was 1.0% and 2%, respectively. The red pigment formation was not influenced by the presence or absence of various vitamins such as thiamine, pyridoxal, p-aminobenzoic acid, panthothenic acid and nicotinic acid.
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