Abstract
It has been shown that the rate of lipolysis and proteolysis differs significantly between red clover genotypes with different levels of polyphenol oxidase (PPO) activity (Lee et al. 2004). Sullivan and Hatfield, (2006) reported the development of genetically modified isolines of red clover with the PPO1 gene silenced. This material was used to examine the role of the red clover PPO enzyme on lipolysis and ultimately C18 polyunsaturated fatty acid biohydrogenation in batch culture. If the role of PPO in reducing ruminal lipolysis of plant lipids is proven it would influence breeding strategies for forages which exhibit this trait in an attempt to increase the levels of beneficial PUFA and decrease detrimental trans and saturated fatty acids in animal products.
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