Red cell alloantibodies in multiple transfused thalassaemia patients

Abstract

Background: Thalassaemia major patients require lifelong transfusion support due to which they are prone for a1loimmunization to foreign RBCs. Alloimmunization can he prevented by extended phenotype match blood transfusion. The study was conducted to know the extent of problem of alloimmunization and to find important red cell antibodies in thalassaemia patients. Methods: A cross-sectional study was conducted. A total of 32 thalassaemia patients were enrolled. The specimen was subjected to red cell alloantibody and autoantibody by column gel agglutination technique. R 1 wR 1R 2R 2, rr (papaine and non papain) and 11 cell panel reagent cells were used in screening and identification of alloantibodies respectively. Result: Six (18.8 %) subjects were alloimmunized. All alloimmunized subjects were recipient of more than 20 units of transfusion. Total seven clinically significant alloantibodies were identified. Anti E and anti c were commonest antibodies in four (12.5%) patients. Conclusion: Red cell alloimmunization is an important risk in thalassaemia patient. 71.4% of alloantibodies were anti E and anti c type. Extended phenotype match blood transfusion for Rh-c, and Rh-E antigens or level 2 antigen matching stringency needs to be explored in preventing alloimmunization in thalassaemia patients.