Red Cabbage Rather Than Green Cabbage Increases Stress Resistance and Extends the Lifespan of Caenorhabditis elegans.

Pu Jing,Nan Zhang,Shunshan Jiao

doi:10.3390/antiox10060930

Pu Jing, Nan Zhang + Show 1 more

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https://doi.org/10.3390/antiox10060930

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Abstract

Many studies have demonstrated that cabbages possess various biological activities, and our previous studies confirmed that cyanidin-3-diglucoside-5-glucoside (CY3D5G), the major core of red cabbage anthocyanins, exhibited in vitro antioxidant activity. This study further investigated the protective effects of CY3D5G derivative from red cabbage juice (RCJ) on oxidative stress and lifespan in cells and Caenorhabditis elegans, green cabbage juice (GCJ) was used as control. RCJ rather than GCJ significantly improved cell viability and decreased lactate dehydrogenase release in H2O2-induced caco-2 cells. RCJ significantly increased survival during oxidative and heat stress and mean lifespan in C. elegans by 171.63% and 31.64%, and 28.16%, respectively, while GCJ treatment showed no significant effects (p < 0.05). These results might be attributed to significantly (p < 0.05) higher contents of total phenolics, ascorbic acid, glucosinolates, and anthocyanins in RCJ compared to those in GCJ. Additionally, both of them decreased autofluorescence and reproductive capacity, increased body length, but did not alter the intracellular ROS level. Prolonged lifespan by RCJ might require heat-shock transcription factor pathway, sirtuin signaling, and calmodulin kinase II pathway, independent of insulin/insulin-like growth factor-1 signaling pathway. RCJ showed promising antioxidant properties in caco-2 cells and C. elegans, which provided more information on the health benefits of cabbage.

Highlights

red cabbage juice (RCJ) treatment did not extend the lifespan of sir-2.1(ok434) worms (p < 0.05 by the log-rank test; Figure 7C), indicating that sir-2.1 was required for the longevity effect of RCJ
RCJ treatment significantly down-regulated the transcription level of osr-1 (p < 0.05), but did not alter sek-1 mRNA level (Figure 7A). These results implied that the OSR-1/UNC-43 pathway was a target for RCJ in C. elegans
Our results revealed that RCJ treatment significantly decreased autofluorescence, while green cabbage juice (GCJ) treatment had no impact on the accumulation of autofluorescent materials in worms

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Aging is a universal and inevitable biological phenomenon. According to the free radical theory of aging, oxidative stress damages various cell components and activates specific signal pathways, which influences numerous cellular processes linked to aging [1]. Supplementation of antioxidants has been provided to reduce the oxidative stress level in model organisms [1]. The optimal source of antioxidants might come from diet. Diet has significantly impact on physiological well-being, and high intake of vegetables has been proved to be beneficial in aging and age-related diseases [2]

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