Recycling of slaughterhouse waste cattle rumen fluid for biohydrogen production using Staphylococcus sciuri MK898925.1

Abstract

ABSTRACT Environmental damage is caused by the waste produced by slaughterhouses. The leftovers from slaughterhouses make a solid base for the production of clean energy. In the present study, biohydrogen was produced using the cattle rumen fluid (RF) collected from the slaughterhouse. The RF serves as the only substrate, and 21.13 ± 0.176 µmol H2 ml−1rumen fluid (RF) was found to be produced during biohydrogen synthesis. After 72 h, the rumen fluid was collected and a pure culture of bacteria that produce biohydrogen was isolated. After characterization, the organism was identified as Staphylococcus sciuri MK898925.1. The Sterilised RF was used as the sole substrate for biohydrogen production by Staphylococcus sciuri MK898925.1, and biohydrogen production was found to be 142.4 ± 2.37 µmol H2 ml−1 RF. Staphylococcus sciuri MK898925.1 was screened for the presence of the gene hydrogenases and it was discovered that the gene was present in the bacteria. The bacteria Staphylococcus sciuri MK898925.1 isolated from the rumen fluid of cattle can be used to produce biohydrogen. A large amount of slaughterhouse waste rumen fluid can be recycled and converted into energy at a very low cost.