Abstract
It is commonly considered not desirable to use microcarriers more than once in the cultivation of anchorage-dependent animal cells. However, our experiment contradicts this belief. The collagen-coated microcarriers, Cytodex-3, from a batch culture of Vero cells, were collected, cooled to 4, agitated in basic phosphate-buffered solution to detach the cells, and then fully washed to remove the cell debris. The microcarriers were then re-applied in cell culture. The rate of cell attachment, growth and metabolism on re-used carriers were found to be comparable to that of on new ones.
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