Abstract
In this study, we analyzed the expression profile of four genes (CCNA2, CCNB1, CCNB2, and CDK1) in laryngeal squamous cell carcinoma (LSCC) cell lines and tumor samples. With the application of microarray platform, we have shown the overexpression of these genes in all analyzed LSCC samples in comparison to non-cancer controls from head and neck region. We have selected CDK1 for further analysis, due to its leading role in cell cycle regulation. It is a member of the Ser/Thr protein kinase family of proven oncogenic properties. The results obtained for CDK1 were further confirmed with the application of reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique, Western blot, and immunohistochemistry (IHC). The observed upregulation of CDK1 in laryngeal squamous cell carcinoma has encouraged us to analyze for genetic mechanisms that can be responsible this phenomenon. Therefore, with the application of array-CGH, sequencing analysis and two methods for epigenetic regulation analysis (DNA methylation and miRNA expression), we tried to identify such potential mechanisms. Our attempts to identify the molecular mechanisms responsible for observed changes failed as we did not observe significant alterations neither in the DNA sequence nor in the gene copy number that could underline CDK1 upregulation. Similarly, the pyrosequencing and miRNA expression analyses did not reveal any differences in methylation level and miRNA expression, respectively; thus, these mechanisms probably do not contribute to elevation of CDK1 expression in LSCC. However, our results suggest that alteration of CDK1 expression on both mRNA and protein level probably appears on the very early step of carcinogenesis.Electronic supplementary materialThe online version of this article (doi:10.1007/s13277-016-4991-4) contains supplementary material, which is available to authorized users.
Highlights
Larynx squamous cell carcinoma (LSCC) is one of the most frequent types of head and neck cancer [1]
The pyrosequencing and miRNA expression analyses did not reveal any differences in methylation level and miRNA expression, respectively; these mechanisms probably do not contribute to elevation of CDK1 expression in laryngeal squamous cell carcinoma (LSCC)
No statistically significant difference between cell lines derived from primary and recurrence tumors was observed, but the CDK1 gene expression level was higher in Quantitative real-time PCR
Summary
Larynx squamous cell carcinoma (LSCC) is one of the most frequent types of head and neck cancer [1]. Several oncogenes have been shown to be involved in the development of head and neck cancers including CCND1, Tumor Biol. CDK1 (cyclin-dependent kinase 1) gene is an important factor of the cell cycle control system [7, 8]. CDK1 is required for mammalian cell proliferation as it is the only CDK that can initiate the onset of mitosis [11] For all these genes, we observed gene upregulation in LSCC cell lines and tumor samples as compared to noncancer controls. Due to the leading role of CDK1 in cell cycle control and regulation, we have chosen the CDK1 for further analysis This gene is a member of the Ser/Thr protein kinases family. The CDK1 protein is a catalytic subunit of M-phase promoting factor essential for the G1/S and G2/M transition in eukaryotic cell cycle
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
