Abstract

Abstract Recurrent backcrossing was used to incorporate the zero-erucic acid allele from a brown-seeded Canadian Brassica campestris cultivar, Tobin, into a high-erucic acid, white-flowered, Yellow Sarson (B. campestris), line M-91. Seeds heterozygous for erucic acid were identified by the half-seed technique and subsequently grown for back-crossing or selfing. Backcrossing, followed by selfing, led to the production of both zero- and high-erucic acid, yellow-seeded, yellow-or white-flowered Yellow Sarson types. Agronomically, the zero- and high-erucic acid genotypes were comparable to the recurrent parent. These results suggested that the half-seed technique can be used to identify those seeds heterozygous for erucic acid alleles, after which the zero- or high-erucic acid alleles can be incorporated into Brassica oil crop cultivars by recurrent backcrossing.

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