Abstract
This study presents a general screening method for the detection of doping agents, including 16 anabolic steroids and 18 glucocorticoids in the human urine samples by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Sample preparation was done based on the single solid phase extraction cartridge of hydrolyzed human urine to produce extraction, and two kinds of solvent-methanol and dichloromethane mix solutions were collected individually. Then the assay was performed by LC-MS/MS in positive ionization mode using an electrospray ionization and multiple reaction monitoring as the acquisition mode. All compounds showed good reproducibility in terms of the relative retention times (both within-day and between-day precision CV% ＜ 1.0). The limit of detection (LOD) was in the range of 1-30 ng/mL for glucocorticoids and anabolic steroids. The overall performance of this method suggests that it is supposed to apply for a routine anti-doping analysis, especially when there is considerable number of samples during competitions.
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