Abstract
Chloroform or antibiotic treated sewage and effluent samples were inoculated directly onto MK 2 or Vero cell monolayers for enterovirus recovery. The results obtained showed a high enterovirus recovery. The average PFU (100 ml) −1 from sewage samples was 4496 and from effluent samples was 5684. The conventional ratio of enteroviruses to coliforms in sewage is considered to be 1:65,000. By the direct inoculation method the ratio obtained was 1: 20,000 for sewage and 1: 2000 for effluent. It is believed the number of enteroviruses in sewage to be even higher than the ones obtained.
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