Abstract
We have recovered transgenic chrysanthemum plants on a medium containing hygromycin after Agrobacterium-mediated transformation. We used the disarmed hypervirulent strain EHA101 containing a binary vector carrying the neomycine phosphotransferase II, the glucuronidase (GUS) and the hygromycin phosphotransferase genes. Different protocols of transformation are ddiscussed. Optimal conditions were inoculations of nodes (dropping 20 min in the bacterial suspension) which were immediately transferred on a regeneration medium containing cefotaxime (500 μg/ml) and hygromycin (10μmg/ml). Neoformation of buds occured on selective medium within two months on almost 40% of the inoculated explants. DNA analysis of plants showing GUS expression confirmed the T-DNA integration in the plant genome.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
