Recovery of structure and activity of disintegrated aerobic granular sludge after long-term storage: Effect of exogenous N-acyl-homoserine lactones

Abstract

Long-term storage of aerobic granular sludge (AGS) may lead to granule inactivation and disintegration. Granule recovery in both structure and activity is important for scale-up and stability of AGS, but information about the structure recovery of stored AGS is limited. In addition, whether short-term exogenous N-acyl-homoserine lactones (AHLs) regulations could accelerate the granule recovery and sustain positive effects on AGS is unknown. Herein, the recovery of 33-month stored AGS was performed in three reactors for 38 days (phase I) at different exogenous AHLs concentrations (0, 50 and 500 nM of AHL-mixtures in R0, R1 and R2, respectively) and for an extended 45 days without exogenous AHLs (phase II). Results demonstrated successful recovery of disintegrated AGS in all reactors, although it was relatively time-consuming in R0. The treatment performance was similar among the reactors and steady-state removal of COD (90%) and NH4+-N (94%) could be recovered within 7 and 21 days, respectively. However, exogenous AHLs regulation (especially in R1) obviously accelerated bioactivity recovery of heterotrophs and nitrifiers and improved granule characteristics, including biomass, density, hydrophobicity and extracellular polymeric substance (EPS). During phase II, sustainable positive effects remained in R1, but granule characteristics deteriorated in R2. The abundance of functional genera Thauera, Nitrosomonas and Candidatus_Nitrotoga, contributed to the rapid recovery and helped maintain the structure and activity of AGS. The predictive functional profiling of bacterial communities also demonstrated sustainably higher activities of metabolism, growth and signal sensing under exogenous AHLs regulation at an appropriate content.