Abstract
A procedure for the quantitative recovery of permeabilized H35 hepatoma cells using lysolecithin is reported. More than 80% of the cells of confluent cultures can be made permeable, which can then seal in defined medium over the next 24 h with essentially complete recovery. Dividing cultures are more susceptible to lysolecithin than confluent cultures. By reducing the concentration of lysolecithin and time of exposure, permeabilized cell preparations can be generated from dividing cultures that will seal when incubated in defined medium supplemented with serum and folinic acid.
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