Abstract
ABSTRACTThe separate steps of preenrichment in lactose broth and selective enrichment in tetrathionate (TET) or selenite‐cystine (SC) broths were combined into single procedures for the detection of both injured and uninjured salmonellae in foods. Following 4 hr of incubation, iodine or selenite were added to combined lactose‐tetrathionate (CTET) or selenite basal broths, forming selective environments. CTET broth was as efficient as conventional TET and SC broths but the combined selenite media were not. Salmonellae were detected in foods in 30 hr using direct fluorescent antibody and combined enrichment techniques. The combined procedure resulted in savings of assay time, labor and materials.
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