Recovery of Salmonellae from Broiler Carcasses by Direct Enrichment

Abstract

Each of four serotypes of Salmonella (S. anatum, S. montevideo, S. saint-paul, S. typhimurium), inoculated at low levels on broiler carcasses (ca. 20 cells/carcass) was detected by direct enrichment of the whole carcass rinse fluid with either Selenite Cystine Broth (SC) or Selenite Brilliant Green Broth (SGB). Neither Selenite Brilliant Green Sulfa Broth (SBGS) nor TT Broth was effective in detecting the serotypes unless the entire broiler carcass with the rinse fluid was incubated with either of these enrichment broths. SBG and SC were effective as direct enrichment broths for recovering pure cultures of the four seroytpes subjected to sublethal heat treatment (53 C for 1 min) approximating that to which broiler carcasses are subjected during the commercial scalding process.