Abstract
NAtural Deep Eutectic Solvents (NADES) were discovered in 2011 as being mixtures of polar “primary metabolites” that exhibit unexpected solubilizing and stabilizing abilities for hydrophobic and/or bioactive ingredients. However, due to the inherent low vapor pressure of NADES, it is challenging to recover analytes such as “secondary” metabolites from a NADES-analyte(s) matrix by conventional liquid chromatography. The present study shows that countercurrent separation (CCS) can perform this task successfully. The resolution capability of CCS depends on the differential distribution coefficients (K values) of the analytes. Compared to most bioactive constituents, the NADES components have extreme K values (close to 0 or ∞, in RP or NP, resp.) because of their high polarity. Glucose-choline chloride-water (2:5:5, mole/mole) with rutin, quercetin, kaempferol, or daidzein were chosen as the test matrices. The CCS fraction analysis by UV-UHPLC and qHNMR showed that CCS can recover the target analyte completely from the NADES-analyte matrix, and at the same time yield the NADES quantitatively, allowing further study of “primary” NADES vs. bioactive “secondary” metabolites in botanical extracts. *represents equal contribution to this work
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
