Abstract
A Ficoll-gradient method to recover separately lymphocytes and sedimented cells from uterotubal flush fluid of cattle is described. Lymphocytes could be cocultured for virus isolation or used for histopathological studies, or both. Epithelioid cells, most likely of oviductal origin, were grown in monolayer cultures to initiate cell lines and isolate viruses. This technique could be applied to other situations that require the growth of bovine oviduct epithelial cells in vitro.
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