Abstract
The ability of lactic cultures to grow on Petrifilm™ SM plates was studied. Frozen yogurt mix was analyzed microbiologically by plating on TOE, LBS, M17 and Petrifilm™ SM. Plates were incubated aerobically in a Gas-Pak System and under a CO2 environment. Also, Lactobacillus bulgaricus, Streptococcus thermophilus, Lactococcus lactis subsp. diacetylactis, and Leuconostoc cremoris were isolated from yogurt and buttermilk samples for study. Isolated cultures were grown in nutrient broth and plated on Petrifilm™ SM and M17 agar. Plates were incubated aerobically and in a Gas-Pak system. Petrifilm™ SM plates performed as well or better than the M17 agar in assaying lactic growth with the exception of Streptococcus thermophilus culture in an aerobic environment. Petrifilm™ SM plates show promise as a method for enumerating viable lactic cultures if incubated in a reduced oxygen environment.
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