Abstract
The objective of this study was to identify whether the efficacy of extracting hesperidin and narirutin from Citrus unshiu peel by-products can be increased by combining pulsed electric field (PEF) and subcritical water extraction (SWE). The samples were treated with a PEF at a strength of 3kV/cm for 60 and 120s. Subsequent SWE was conducted at extraction temperatures of 110-190°C for 3-15min. The concentration of hesperidin was highest at 46.96 ± 3.37mg/g peel (dry basis) after PEF treatment at 120s, combined with SWE at 150°C for 15min, while that of narirutin peaked at 8.76 ± 0.83mg/g after PEF treatment at 120s, integrated with SWE at 190°C for 5min. The concentrations of both hesperidin and narirutin increased with PEF treatment time. The PEF increased the amounts of hesperidin and narirutin extracted by 22.1% and 33.6%, respectively. This study demonstrate the potential of PEF pretreatment for enhancing the SWE of flavonoids from C. unshiu peel.
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