Abstract
The growth kinetics of haemopoietic cells after irradiation was compared between the normal (+/+) and the genetically anemic W/Wυ mice both in vivo and in vitro. The parameters studied were the total cellularity and the number of granulocyte/macrophage precursor cells (CFUc). The onset of haemopoiesis in vivo after irradiation with 150R of X-rays was significantly delayed in W/Wυ mice compared to that in +/+ littermates. In the culture of W/Wυ marrow, haemopoietic cells could recover after 50R-irradiation but not after irradiation with more than 100R, whereas the +/+ marrow culture restored normal haemopoiesis soon after irradiation. The number and concentration of CFUc within the W/Wυ marrow culture showed a temporary ncrease after irradiation, followed by a gradual decrease which preceded the depletion of differentiated cells in suspension. These results suggest that the differentiation potency of W/Wυ stem cell is normal but the capacity for self-renewal is defective.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
