Abstract
AbstractCytokinins exhibit great affinity for the polystyrene matrix of Dowex‐50 cation exchange resins. Model experiments showed that only c. 20% of the applied 2‐methylthio‐trans‐zeatin and c. 70% of the trans‐zeatin are eluted from AG 50W columns with six bed volumes of 1 N NH4 OH. Inclusion of 70% (v/v) ethanol in the eluant increases the recovery to c. 55 and 85% respectively. No additional recovery was obtained upon continued elution to 14 bed volumes. Similar experiments with Duolite CS‐101, a weak acid cation exchange resin with acrylic matrix, showed that c. 95% of the applied 2‐methylthio derivatives of isopentenyladenine and zeatin are recovered from the resin with six bed volumes of 70% (v/v) ethanol (pH 10) or 1 N NH4OH in 70% (v/v) ethanol. The less modified cytokinins, isopentenyladenosine and ribosylzeatin, are recovered completely under these conditions. Recovery of cytokinins was determined by means of high‐pressure liquid chromatography of the column eluates.
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