Recovery of antibacterial sensitivity of ESBLs-producing fowl <I>E. coli</I> by sulbactam and EDTA

Abstract

To determine effect of sulbactam and EDTA on antibacterial activity of antimicrobial drugs in vitro,MIC of 10 antimicrobial agents alone and their respective combinations with sulbactam,EDTA and sulbactam+ EDTA against ESBLs-producing E.coli,were determined by using broth microdilution method.The results showed that sulbactam and EDTA can enhance antibacterial activity of parts of antimicrobial agents,the resistant rate of ESBIs-producing isolates to third generation cephalosporin ceftiofur,cefotaxime was higher than 80% and the resistant rates to fluoroquinolones,doxycycline,florfenicol,amikacin and fosfomycin were up to 100%.All the ESBIs-producing isolates were sensitive to cefepime.The resistant rate to third generation cephalosporin cefotaxime,florfenicol,amikacin and fosfomycin combinations with sulbactam,were lower than that to antibacterial drugs alone,is 0,88.9%,88.9%,0 respectively.The resistant rate to gatifloxacin,doxycycline,florfenicol,amikacin and fosfomycin combinations with EDTA,were lower than that to antibacterial drugs alone,is 30%,11.1%,22.2%,11.1%,0 respectively.The resistant rates of most strains to sulbactom+EDTA amikacin,fosfomycin,florfenicol and ceftiofur combinations were much lower than those combinations either only sulbactam or EDTA and were all 0.