Recovery of a Bacteriocin-Like Inhibitory Substance from Lactobacillus bulgaricus FTDC 1211 Using Polyethylene-Glycol Impregnated Amberlite XAD-4 Resins System.

Nur Fazrin Husna Abdul Aziz,Sahar Abbasiliasi,Joo Shun Tan,Arbakariya Bin Ariff,Mazni Abu Zarin,Siti Nurbaya Oslan,Zhang Jin Ng

doi:10.3390/molecules25225332

Nur Fazrin Husna Abdul Aziz, Sahar Abbasiliasi + Show 5 more

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https://doi.org/10.3390/molecules25225332

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Abstract

Lactobacillus bulgaricus is a LAB strain which is capable of producing bacteriocin substances to inhibit Staphylococcus aureus. The aim of this study was to purify a bacteriocin-like inhibitory substance (BLIS) produced by L. bulgaricus FTDC 1211 using an aqueous impregnated resins system consisting of polyethylene-glycol (PEG) impregnated on Amberlite XAD4. Important parameters influencing on purification of BLIS, such as the molecular weight and concentration of PEG, the concentration and pH of sodium citrate and the concentration of sodium chloride, were optimized using a response surface methodology. Under optimum conditions of 11% (w/w) of PEG 4000 impregnated Amberlite XAD4 resins and 2% (w/w) of sodium citrate at pH 6, the maximum purification factor (3.26) and recovery yield (82.69% ± 0.06) were obtained. These results demonstrate that AIRS could be used as an alternate purification system in the primary recovery step.

Highlights

Staphylococcus aureus skin infections pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates [1]
The cell culture free supernatant (CCFS) from L. bulgaricus FTDC 1211 was used for subsequent experiments
bacteriocin-like inhibitory substance (BLIS) from L. bulgaricus FTDC 1211 was partially purified in this study

Summary

Introduction

Staphylococcus aureus skin infections pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates [1]. As an alternative mode of treatment, bacteriocins have been shown to possess antimicrobial efficacy against multiple drug resistant strains. This reveals the huge potential of bacteriocin as an alternate drug in the pharmaceutical industry. Bacteriocins are ribosomally synthesized, antimicrobial peptides or proteins produced by various microorganisms which have the ability to inhibit the growth of bacteria with closely related species. The most studied bacteriocin-producing microorganisms are lactic acid bacteria (LAB), which have the status of generally recognized as safe (GRAS) microorganisms [2].

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