Recovery in Function in Olfactory Escheating Cell Transplanted Rat Spinal Cord After Stopping the Injection of Cyclosporine

Abstract

The study aimed to check whether animals can retain directed forepaw reaching (DFR) function after stopping cyclosporine which is used to prevent immunorejection in xenotransplant of Olfactory Encheating cells (OECs) in Albino Swiss Strain (AS) rat corticospinal tract lesion. The Hematoxylin & Eosin (H&E) staining and immunohistochemistry techniques have been performed on the cryostat sections of corticospinal tract (CST) lesion post-surgery to check the immunorejection and the existence of OEC transplanted tissue before and after injection of cyclosporine. The DFR function have been checked after each stage to see whether animals retain the DFR tasks after stopping cyclosporine . The results show that the daily injection of 100ml diluted cyclosporine avoids immune attack and reduces inflammatory cells infiltrate in delayed CST ipsilateral rat model and also our study proved that stop of cyclosporine injection would not prevent DFR function and the regenerated fibres can still be visualised after 6 months post-operation period.Despite the fact that so many methods such as animal genetic engineering is used to avoid imminuregection after xenotrasplantation in animal model, daily cyclosporine injection for some period after surgery seems to be effective and effortless method to not only deter immunorejection in host animal but it also preserve function such as DFR for a long period post-surgery