Abstract
In this study, muscle-derived stem cells (MDSCs) whose differentiation into neuron-like cells was induced by ciliary neurotrophic factor (CNTF) and Salvia (Salvia miltiorrhiza) in vitro were used to repair rat sciatic nerve injuries in vivo, in order to investigate their multifunctional characteristics as pluripotent stem cells. The sciatic nerve in the right side of the lower limb was exposed under the anesthetized condition of 10% chloral hydrate (0.3 ml/100 g) injection into the abdominal cavity. The tissue which was 0.5 cm above the sciatic nerve bifurcation was broken using a hemostat. After induction, MDSCs were transferred in sodium hyaluronate gel and were placed into the damaged area. An untreated control group was also included in this study. The surgical area was sutured after washing with gentamycin sulfate solution. Sciatic nerve function index (SFI) was calculated, electrophysiological tests were performed and the recovery rate of gastrocnemius muscle wet weight was also calculated. Four weeks post-surgery, the SFI and the recovery rate of gastrocnemius muscle wet weight in the MDSC group were significantly higher than those in the control group (P<0.05). MDSCs whose differentiation is induced by CNTF and Salvia play an active role in the repair of peripheral nerve injury.
Highlights
Several researchers have performed numerous studies in this field and proposed that the basic condition for peripheral nerve repair is the construction of the nerve regenerative chamber, which can create a specific microenvironment for regeneration of the nerve (1,2)
As the study of Chinese traditional medicine has developed, it has been confirmed that Salvia (Salvia miltiorrhiza) may be used to induce the differentiation of multi-functional stem cells into neuron-like cells (7-10)
There were no obvious differences between the Muscle-derived stem cells (MDSCs) and control groups
Summary
Several researchers have performed numerous studies in this field and proposed that the basic condition for peripheral nerve repair is the construction of the nerve regenerative chamber, which can create a specific microenvironment for regeneration of the nerve (1,2). As stem cell research has developed, study into novel tissue engineering seed cells has received increasing attention. Muscle-derived stem cells (MDSCs) originate from muscle tissue. In addition to aspects shared by stem cells, MDSCs exhibit such characteristics as harmlessness to the body, a wide range of sources, ready acceptance by patients, lack of restrictions of source of donor, lack of immunosuppressive effects and fewer ethical restrictions. MDSCs are ideal seed cells for peripheral nerve repair (3-6). As the study of Chinese traditional medicine has developed, it has been confirmed that Salvia (Salvia miltiorrhiza) may be used to induce the differentiation of multi-functional stem cells into neuron-like cells (7-10)
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