Recovery ability of sperm after freezing of pasundan bull semen

Abstract

The purpose of this study is to look at Pasundan bulls’ sperm-freezing capacity for recovery. Three bulls’ sperm were collected using an artificial vagina. According to the normal operating protocols of the Center for Artificial Insemination Breeding and Development of Beef Cattle, Ciamis, West Java, fresh semen with sperm motility of 70% was frozen using a tris egg yolk extender. Evaluations of the percentage of motility, viability, and plasma membrane integrity were done under fresh, equilibrium, and post-thawing conditions to determine sperm destruction and the capacity of sperm to recover in each phase. Using the statistical package SPSS Version 25 application, descriptive statistics were used to examine all of the data. The findings indicated that the percentages of motility, viability, and plasma membrane integrity decreased during the freezing procedure. The motility, viability, and plasma membrane integrity of pasundan bovine semen recovered at rates of 64.29, 78.97, and 63.13%, respectively, following the freezing procedure. Based on recovery rates, it is demonstrated that pasundan bull semen has strong resistance and successful survival rates throughout the freezing procedure.