Recovered Gases and Bacteria from the &lt;i&gt;Clostridium&lt;/i&gt; Genus as Ecological Condition Indicators of Bottom Sediments of the Eastern Donbass Small Rivers

Roman Gennad'Yevich Trubnik,Ludmila Anatol'Yevna Nedoseka,Yuriy Aleksandrovich Fedorov

doi:10.3844/ojbsci.2017.201.210

Recovered Gases and Bacteria from the <i>Clostridium</i> Genus as Ecological Condition Indicators of Bottom Sediments of the Eastern Donbass Small Rivers

Roman Gennad'Yevich Trubnik, Ludmila Anatol'Yevna Nedoseka + Show 1 more

Open Access

https://doi.org/10.3844/ojbsci.2017.201.210

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Abstract

The results of determination of methane, total hydrogen sulfide and sulfite reducing clostridia in bottom sediments of small rivers and reservoirs of the Eastern Donbass are presented. The horizontal and vertical distribution of levels of reduced gases in bottom sediments is analyzed. The ecological condition of the bottom sediments was estimated by the clostridia titer and the methane index. The ranking of the ecological condition of bottom sediments according to the content of total hydrogen sulfide in them is performed. The relationship between pH and Eh values, methane and hydrogen sulfide concentrations and the number of sulfite reducing clostridia was studied, which allowed to discover new sides of the metabolic cycle of reduced gases in bottom sediments of freshwater ecosystems located in the zone of anthropogenic influence. The researches carried out allow to draw a conclusion about significant prolonged anthropogenic impact, mainly of domestic and industrial wastewater on water objects of the Eastern Donbass.

Highlights

The investigations were carried out on small rivers of the Eastern Donbass Table 1, Fig. 1
The results of the study demonstrated that the vegetative cells of sulfite-reducing clostridia are widely distributed within all investigated water objects, exceeding the normative indices by the titer of clostridia by two or more orders
The results of estimating bottom sediments by the content of methane and total hydrogen sulfide indicate the allochthonous intake of organic substances in water bodies and waterways of domestic and industrial wastewater

Summary

Introduction

The investigations were carried out on small rivers of the Eastern Donbass Table 1, Fig. 1. This region is located in the South of the European territory of Russia in Rostov region and it is the main coal resource base of the Southern Federal District. The life activity of clostridia is carried out under anaerobic conditions and is accompanied by the production of toxins, enzymes and proteins that determine gas formation and necrosis in tissues, as well as a general toxic effect on the human body, animals and hydrobionts (Bylova et al, 2011; Shevchenko et al, 2013; Morozova and Fedorov, 2015).

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Conclusion