Abstract

A novel application of holographic interferometry to study the development of behaviour of an avian embryo in an intact incubating egg is reported. A holographic system is implanted astribe both the interior and exterior of the incubator. The observation of the movements of the embryo is obtained in the form of the evolution of fringes in real-time by means of a vidicon camera placed behind the holographic plate. Besides being useful in the study of the pre-natal motility cycle, the method allows one to follow the development of specific behaviour patterns, their onset, duration and frequency of repetition. The steady development of the intense movements leading to the first cracking of the shell is vividly displayed. Preliminary results confirming the experimental viability of the proposed application are presented.

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