Recording electric potentials from single adherent cells with 3D microelectrode arrays after local electroporation

Abstract

This short communication reports on the innovative method of the local micro-invasive needle electroporation (LOMINE) of single adherent cells. The investigation of cellular reactions in living cell cultures represents a fundamental method, e.g. for drug development and environmental monitoring. Existing classical methods for intracellular measurements using, e.g. patch clamp techniques are time-consuming and complex. Present patch-on-chip systems are limited to the investigation of single cells in suspension. Nevertheless, the most part of the cells of the human body is adherently growing. Therefore, we develop a new chip system for the growth of adherent cells with 64 micro-structured needle electrodes as well as 128 dielectrophoretic electrodes, located within a measuring area of 1 mm 2. With this analytical chip, the intracellular investigation of electro-chemical changes and processes in adherently growing cells will become possible in the near future. Here, we present first intracellular measurements with this chip system.