Record of Caromiobenella (Copepoda, Monstrilloida) in Brazil and Discovery of the Male of C. brasiliensis: Morphological and Molecular Evidence

Abstract

Monstrilloid copepods are protelean parasites with a complex life cycle that includes an endoparasitic juvenile phase and free-living early naupliar and adult phases. The monstrilloid copepod genus Caromiobenella Jeon, Lee and Soh, 2018 is known to contain nine species, each one with a limited distribution; except for two species, members of this widespread genus are known exclusively from males. Hitherto, members of Caromiobenella have not been recorded from tropical waters of the South Western Atlantic (SWA). The nominal species Monstrilla brasiliensis Dias and Suárez-Morales, 2000 was originally described from female specimens collected in coastal waters of Espírito Santo and Rio de Janeiro (Brazil), but the male remained unknown. The failure to reliably link both sexes of monstrilloid species is one of the main problems in the current taxonomy of the group, thus leading to a separate treatment for each sex. New zooplankton collections in coastal waters and intertidal rocky pools of the SWA yielded several male and female monstrilloid copepods tentatively identified as Monstrilla brasiliensis. Our results of both morphologic and molecular (mtCOI) analyses allowed us to confirm that these males and females were conspecific. We also found evidence suggesting that Caromiobenella is not a monophyletic taxon. Our male specimens are morphologically assignable to Caromiobenella, therefore, females of the nominal species Monstrilla brasiliensis, are matched here with the aforementioned males and, thus, the species should be known as C. brasiliensis comb. nov. (Dias and Suárez-Morales, 2000). This finding represents the third documented discovery of a female of Caromiobenella, the first record of the genus in the Southwestern Atlantic, and the first documented record of monstrilloids from coastal tidepools. With the addition of C. brasiliensis, Caromiobenella now includes 10 valid species worldwide. This work represents the second successful use of molecular methods to link both sexes of a monstrilloid copepod. The male of C. brasiliensis is herein described, and a key to the known species of Caromiobenella and data on the habitat and local abundance of C. brasiliensis are also provided.