Abstract
Electron microscopic studies on proteins require solution of the following problems: 1/ when preparing the samples one strives for preservation of structure and its better revelation by staining; 2/ elect ron microscopy at maximal resolution and with minimal radiation damages; 3/ interpretation of images, their processing through optical and mathematical methods. All these problems are inseparably linked with one another, therefore, while concentrating attention on point 3, we shall also touch upon the first two.Until now, negative staining has been the most effective method for enhancing contrast of protein molecules and protecting structure during drying. For the sake of simplicity, the molecule images on micrographs may be regarded as patterns of stain distribution around the structure. In reality, the fine details around the edges of protein molecules can be lost in the depths of stain.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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