Abstract
BackgroundThe common formation of images in CSLM assumes mechanically scanned object placed in the common short focus of the objective lenses of the microscope, while in the arrangement under study, the scanning of the object is realized by placing a diffuser behind the collimating lens. A model is suggested in the formation of images in Confocal Scanning Laser Microscope (CSLM) using non-scanned object. Since the illumination and detection are coherent, the obtained image is constructed from the simple product of the Resultant Point Spread Function (RPSF) modulated by the diffuser spread over the object transparency. Hence, the product of the object and the image of the diffuser replace the mechanical scanning of the object.ResultsReconstructed images using this novel arrangement of CNSM are presented using mammographic X-ray image.ConclusionsConvolution of the RPSF and the object is realized by the spreading of the diffuser image over the object. A coherent detector captures the whole image affected by a noisy diffused function. It is noted that image processing is necessary to improve noisy images making use of filtration techniques.
Highlights
The common formation of images in Confocal Scanning Laser Microscope (CSLM) assumes mechanically scanned object placed in the com‐ mon short focus of the objective lenses of the microscope, while in the arrangement under study, the scanning of the object is realized by placing a diffuser behind the collimating lens
Since the convolution product of the diffuser and the Point Spread Function (PSF) corresponding to the 1st objective lens of aperture P1(x, y) gives truncated image of the diffuser convoluted by the 1st objective PSF formed in the object plane (x, y), we can write the complex amplitude corresponding to the modulated diffuser image as follows: d mod . x, y = d x, y ⊗ h1 x, y
The detected image intensity is affected by the PSF corresponding to the 2nd objective, while the PSF corresponding to the 1st objective is convoluted with the diffuser image giving the modulated diffuser
Summary
The common formation of images in CSLM assumes mechanically scanned object placed in the com‐ mon short focus of the objective lenses of the microscope, while in the arrangement under study, the scanning of the object is realized by placing a diffuser behind the collimating lens. The ordinary confocal laser scanning microscope (CSLM) described early by Sheppard et al [1,2,3,4,5,6,7,8,9,10,11,12] assumes mechanical scanning of the object placed in the common short focus of the objective lenses of the microscope. We are interested to form images using non-scanned confocal microscope making using diffuser placed behind the collimating lens instead of using a grating [18] Previous studies [13,14,15,16,17,18] showed an improvement in the lateral resolution
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Beni-Suef University Journal of Basic and Applied Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
