Reconstruction of bladder with tissue-engineered graft constructed by bone marrow mononuclear cells and PLGA-collagen nanofiber: an animal experiment

Abstract

Objective To investigate the feasibility and function parameters of tissue engineered bladders constructed by bone marrow stem cells seeded on PLGA-collagen nanofiber. Methods New Zealand white rabbit bone marrow mononuclear cells were induced into urinary epithelial cells and smooth muscle cells in vitro cultivation. The cells were seeded on the PLGA-collagen nanofibers as a certain density to form the compound of the nanofiber scaffold carrying the cells. Finally, the compounds were transplanted into the rabbit to carry out bladder repair. Results The morphological observation indicated that the cultured cells could penetrate into the PLGA-collagen nanofibers and form several-layered cell structures. The compound of the cells and the nanofiber scaffold could have angiogenesis. After 6 months, the replacement of bladder capacity determination, cystography examination, urodynamics test had no difference to the normal bladder. And no obvious difference was found in the bladder replacement part and the artificial bladder mucosa with normal bladder by morphological observation and histological observation after HE staining. Conclusions It is a more appropriate and effective engineering bladder replacement tissue with the bone marrow stem cells seeded on the PLGA-collagen nanofibers scaffold. Key words: Urinary Bladder; Stem Cells; Nanostructures; Animal Experimentation